RNA interference : (Record no. 313183)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05478nam a2200721 i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 20093020724 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | CtWfDGI |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20201203121003.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS--GENERAL INFORMATION | |
| fixed length control field | m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr zn||||uuuuu |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 130918t20092009enkad ob 001 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Cancelled/invalid ISBN | 9781845934101 (alk. paper) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Cancelled/invalid ISBN | 1845934105 (alk. paper) |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | CtWfDGI |
| Language of cataloging | eng |
| Description conventions | rda |
| Transcribing agency | CtWfDGI |
| 050 14 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QP623.5.S63 |
| Item number | R55 2009eb |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.8/8 |
| Edition number | 23 |
| 245 00 - TITLE STATEMENT | |
| Title | RNA interference : |
| Remainder of title | methods for plants and animals / |
| Statement of responsibility, etc | edited by Tim Doran, CSIRO Livestock Industries, Australia and Chris Helliwell, CSIRO Plant Industry, Australia. |
| 264 #1 - | |
| -- | Wallingford, Oxfordshire, UK : |
| -- | CABI, |
| -- | 2009. |
| 264 #4 - | |
| -- | ©2009 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (xii, 257 pages) : |
| Other physical details | illustrations, charts |
| 336 ## - | |
| -- | text |
| -- | txt |
| -- | rdacontent |
| 337 ## - | |
| -- | computer |
| -- | c |
| -- | rdamedia |
| 338 ## - | |
| -- | online resource |
| -- | cr |
| -- | rdacarrier |
| 490 1# - SERIES STATEMENT | |
| Series statement | Principles and protocols |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc | The discovery that small RNA molecules act through RNA interference (RNAi) pathways as potent regulators of chromatin structure, messenger RNA (mRNA) abundance and translation, has revolutionized our understanding of gene regulation. These small RNA silencing systems have also been harnessed as powerful tools to manipulate gene expression that can be applied across eukaryotic species. The most frequent application of RNAi by researchers has been functional genomics. In combination with the increasing availability of sequenced genomes, RNAi has provided a powerful method for deciphering gene function by specific targeting of gene expression and subsequent study of the silenced phenotype. This approach has been extremely valuable in eukaryotic species for which traditional gene knockout methods are not easily applied. Gene silencing has been used to understand the function of many plant genes and is also beginning to find applications in agriculture. The researcher has a choice of silencing systems, each with particular advantages and drawbacks. The major factor in determining the silencing system for a particular application is the availability of a stable transformation system for the species being studied. Where a transformation system is available, methods based on long hairpin transgenes and artificial microRNAs (miRNA) can be deployed to best effect. The hairpin transgene and artificial miRNA can also be deployed in transient transfection systems. There are many vectors available to produce the constructs for these systems and web-based tools to assist in selecting the optimum sequences to target a particular gene. Constructs have also been developed that use recombination systems to allow rapid generation of multiple constructs for large-scale functional genomics projects. The chapters of this book cover the use of the three major gene-silencing methods in plants and the application of RNAi in major experimental animal systems. The detailed theoretical background given in each chapter and comprehensive protocols should assist researchers in effective utilization of RNAi technologies. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Hairpin RNAi in plants / Louisa Matthew -- Virus-induced gene silencing (VIGS) to study gene function in plants / Xiaohong Zhu and Savithramma P. Dinesh-Kumar -- The use of artificial microRNAs in targeted gene silencing / John Paul Alvarez and Yuval Eshed -- RNAi in Caenorhabditis elegans / Nicholas M. Johnson, Julie-Anne Fritz and Carolyn A. Behm -- RNAi in Drosophila / Steven Whyard -- RNAi in fish and crustaceans / Mario Pablo Estrada, Juana María Lugo and Yamila Carpio -- RNAi in chicken embryos / Nick J. Van Hateren, Raman M. Das and Stuart A. Wilson -- RNAi in mammalian cells / Yan Zeng -- siRNA and shRNA design / Debra J. Taxman. |
| 506 ## - RESTRICTIONS ON ACCESS NOTE | |
| Terms governing access | Access limited to subscribing institution. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Also available in print format. |
| 588 ## - | |
| -- | Title from PDF title page (viewed August 13, 2013). |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gene silencing. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA, Small Interfering |
| General subdivision | physiology. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA Interference. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Small interfering RNA. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Breeding and Genetics. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal Genetics and Breeding, (New March 2000) |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genetic Engineering, Gene Transfer and Transgenics, (New June 2002) |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular Biology and Molecular Genetics, (Discontinued March 2000, Reinstated and Revised June 2002) |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal breeding. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Animal genetic resources. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Chromatin. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gene expression. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gene silencing. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genes. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genetic engineering. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genomes. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Phenotypes. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant breeding. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Regulatory genes. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA. |
| Source of heading or term | cabt |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA interference. |
| Source of heading or term | cabt |
| 655 #0 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | FF020 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | LL240 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | WW100 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | ZZ360 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Doran, Tim, |
| Titles and other words associated with a name | Dr., |
| Relator term | editor of compilation. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Helliwell, Chris, |
| Relator term | editor of compilation. |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | C.A.B. International, |
| Relator term | issuing body. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Display text | Print version: |
| Title | RNA interference. |
| Place, publisher, and date of publication | Wallingford, UK ; Cambridge, MA : CABI, c2009. |
| International Standard Book Number | 9781845934101 |
| Record control number | (DLC)2008012849 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Principles and protocols. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://dx.doi.org/10.1079/9781845934101.0000">https://dx.doi.org/10.1079/9781845934101.0000</a> |
| Public note | Click here to access resource |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | E-BOOKS |
No items available.