Molecular methods in plant disease diagnostics : principles and protocols / edited by Neil Boonham, Jenny Tomlinson and Rick Mumford.
Material type:
TextPublisher: Wallingford, Oxfordshire, UK : CABI, 2016Copyright date: 2016Description: 1 online resource (xii, 200 pages) : color illustrations, chartsContent type: - text
- computer
- online resource
- Plant diseases -- Diagnosis
- Plant Diseases -- genetics
- Molecular Diagnostic Techniques -- methods
- Viral, Bacterial and Fungal Diseases of Plants, (New March 2000)
- Molecular Biology and Molecular Genetics, (Discontinued March 2000, Reinstated and Revised June 2002)
- Taxonomy and Evolution
- Genetics and Molecular Biology of Microorganisms, (New March 2000)
- Techniques and Methodology
- Detection
- Molecular genetics techniques
- Molecular taxonomy
- Nucleic acids
- Nucleotide sequences
- Plant diseases
- Plant pathogens
- Polymerase chain reaction
- Quality controls
- Taxonomy
- FF610
- ZZ360
- ZZ380
- ZZ395
- ZZ900
- 632.3 23
- SB731 .M58 2016eb
This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification, showing that great strides have been made in molecular diagnostics since those first tentative steps back in the late 1980s/early 1990s. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
Includes bibliographical references and index.
Introduction : advances in plant diagnostics : historical perspectives and future directions / Rick Mumford -- Conventional PCR / Lisa I. Ward, Tracey M. Immanuel, Subuhi Khan, Lia W. Liefting and Catia Delmiglio -- Real-time PCR / Maja Ravnikar, Natas̆a Mehle, Kristina Gruden and Tanja Dreo -- Loop-mediated isothermal amplification (LAMP) / Jenny Tomlinson -- DNA barcoding for identification of plant pathogens / Jennifer Hodgetts -- DNA barcoding of invertebrate plant pests / Sebastian Kiewnick, Andreas Bühlmann and Jürg E. Frey -- Microarrays / Ian Adams, Jenny Tomlinson and Catherine Harrison -- Next-generation sequencing / Rachel Glover and Ian Adams -- On-site testing for plant pathogens / Jenny Tomlinson -- Quality assurance for molecular testing in plant health / Aude Chabirand, Geraldine Anthoine, Adrian Fox and Lynn Laurenson.
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